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dummy Poland service request form dummy


This form is an service of the Biophysics Department (Institut für Physikalische Biologie; Heinrich Heine-Universität Düsseldorf), further informations are available from the help page!
The Poland server will calculate the thermal denaturation profile of double-stranded RNA, DNA or RNA/DNA-hybrids based on sequence input and parameter settings in this form.
NEW: Thermodynamic parameters set for dsDNA in 75 mM NaCl (Blake & Delcourt) added.
Calculation is based on Poland's algorithm in the implementation described by Steger. Graphics results are directly sent to your WWW client.
Sequence title line:
Sequence:
(plain format;
no numbers;
max. 1000 nts;
min. 5 nts)
Mismatched positions: (comma-separated numbers)
Thermodynamic parameters:
Dissociation constant ß:
Oligonucleotide
(ß is function of seq.length) 		Long double strand
(default: ß=1.0E-3/M)
Strand concentration:
(default: 1.0E-6 M)
Temperature range:
Low temperature limit: (default: 40.0 °C) High temperature limit: (default: 110.0 °C) Temperature step size: (default: 2.0 °C)
Which graphics do you want:
Tm(p=50%) plot 3d plot Mobility plot Melting curve Diff. melting curve
Graphics size:
(GIF format)
Click here to , or
Click here to the form to defaults.


 
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Institut für Physikalische Biologie HHU Düsseldorf