POLAND REQUEST FORM: Stuff you'd like to have melted for you.

	Poland service request form

	This form is an service of the Biophysics Department (Institut für Physikalische Biologie; Heinrich Heine-Universität Düsseldorf), further informations are available from the help page!
	The Poland server will calculate the thermal denaturation profile of double-stranded RNA, DNA or RNA/DNA-hybrids based on sequence input and parameter settings in this form.
	Calculation is based on Poland's algorithm in the implementation described by Steger. Graphics results are directly sent to your WWW client.

Sequence title line:

Sequence:
(plain format;
no numbers;
max. 1000 nts;
min. 5 nts)

Mismatched positions: (comma-separated numbers)

Oligonucleotide (ß is function of seq.length)	Long double strand (default: ß=1.0E-3/M)

Strand concentration:
(default: 1.0E-6 M)

Low temperature limit: (default: 40.0 °C)	High temperature limit: (default: 110.0 °C)	Temperature step size: (default: 2.0 °C)

T_m(p=50%) plot	3d plot	Mobility plot	Melting curve	Diff. melting curve

Graphics size:
(GIF format)

Click here to , or
Click here to the form to defaults.


	Institut für Physikalische Biologie (Department of Biophysics) Heinrich Heine-Universität Düsseldorf, Germany

	Sep. 28, 2012
	G. Steger, A. Wilm, S. Gräf, M. Labensky & A. Jäger

Poland service request form