Supplemental data


Matousek, Orctová, Ptácek, Patzak, Dedic, Steger, and Riesner (2007)
Experimental transmission of Pospiviroid populations to weed species characteristic for potato and hop fields.
J. Virol. 81, 11891-11899.

Fig. S1: Predicted thermodynamically optimal secondary structures of PSTVd strains used for infection (boxed) and resulting mutants found in this study. Nucleotides differing from strain Intermediate (1) are given in red. Structure predictions were performed with RNAfold version 1.6.1 (2) at 37°C. Next to the strain or mutant name is given the calculated free energy ΔG°(37°C) of the structure.


(1) Gross, Domdey, Lossow, Jank, Raba, Alberty & Sänger (1978).
Nucleotide sequence and secondary structure of potato spindle tuber viroid.
Nature 273, 203­--208.

(2) Hofacker (2003).
Vienna RNA secondary structure server.
Nucleic Acids Res. 31, 3429­--3431.


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